LifeGlobal® is the Media of the Future
The Future is NOW!
| A Unified
Approach to Human Embryo Culture Don Rieger, PhD and Klaus Wiemer, PhD |
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| Comparisons of
Global with Sequential Media for Culture of Human
Embryos from the Zygote to Blastocyst Stage Zech N, Vansteenbrugge A |
| Comparison of
Two Systems To Culture Human Embryos up to Day 3: A
Prospective Randomized Study S. Verza, 2007 ASRM Abstract |
| Comparison of
Global Medium and GI/G2 cleavage/blastocyst sequential
media for culture of human embryos after IVF E. Greenblatt, T. Di Beraradino, P. Chromis-Brown, D. Holt, A. Lains, 2005 ESHRE abstract |
| Comparisons of
a single medium with sequential media for the
development of human zygotes to the blastocyst stage Vansteenbrugge A, 2007 ASRM abstract |
| A Randomized
Prospective Comparison of a Sequential Versus Single
Media Culture System Dawn Kelk, global versus Cook abstract |
| Comparison of a
Single Medium with Sequential Media for Culture of
Sibling Human Embryos to the Blastocyst Stage S. Sepulveda, J. Garcia, E. Arriaga, L. Noriega, K.E. Wiemer, D. Rieger |
| Clinical
Studies & Development and Use of global® Medium
Don
Rieger, PhD |
| Enhanced effect
of glycyl-L-glutamine on mouse preimplantation emryos in vitro John D Biggers, Lynda K McGinnis, JA Lawitts
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| Mouse embryo
development following IVF in media containing either
L-glutamine or glycyl-L-glutamine
M.C.
Summers, L.K. McGinnis, J.A. Lawitts and J.D. Biggers |
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Comparison of a Single Medium with Sequential Media for Development of Human Embryos To the Blastocyst Stage
Melanie
R. Freeman1 and Don Rieger2 |
 global® Supports High Blastocyst Development Rates and Pregnancy Rates
In recent trials with unselected patients ranging in age from 27-43 years old the ongoing pregnancy rates were 51% with subsequent implantation rates of 27%.2
1. Klaus E. Wiemer, unpublished data, Klaus E. Wiemer, et al. (2001) |
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1. John D. Biggers, Catherine Racowsky. The development of fertilized human ova to the blastocyst stage in medium KSOM AA. Is a two step protocol necessary? Reproductive BioMedicine Online: vol 5. No. 2 133-140 (2002) John D. Biggers, Lynda K. McGinnis, Michael Raffin. Amino Acids and preimplantation development of the mouse in protein-free potassium simplex optimized medium. Biology of Reproduction; 63 281-293 (2000)
Michael C. Summers, Lynda K. McGinnis, Joel A. Lawitts, Michael Raffin and John D. Biggers. IVF of mouse ova in a simplex optimized medium supplemented with amino acids. Human Reproduction vol.15 no.8 pp.1791-1800, (2000) |
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The concentrations of glutamine and ammonia in commercially available cell culture media
The amino acid glutamine is an essential nutrient for cells in culture. In aqueous solutions such as liquid culture media, glutamine spontaneously decomposes into ammonia. In this study, we examined the toxicity of ammonia for two different cell lines. The results suggest that precautions must be taken to avoid the deterioration of commercially available culture media, because of the decay of glutamine. Long storage times lead to a rapid decay of glutamine and an accumulation of the toxic degradation product ammonia.
Heeneman, S., Deutz, N. E. & Buurman, W. A. The concentrations of glutamine and ammonia in commercially available cell culture media. J Immunol Methods 166, 85-91 (1993). |
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Reduction of ammonia formation in cell cultures by L-alanyl-L-glutamine requires optimization of the dipeptide concentration
In cell culture media, glutamine (Gln) decomposes progressively into ammonia and 5-pyrrolidone carboxylic acid. Ammonia is toxic and reduces cell growth. The formation of ammonia is strongly decreased by using the alanine-glutamine dipeptide, L-alanyl-L-glutamine (L-Ala-L-Gln). However, the effects of this dipeptide on the amount of ammonia originating from cellular Gln metabolism are not know. Growing microglial BV-2 cells in a medium containing either Gln or L-Ala-L-Gln showed that the replacement of Gln by L-Ala-L-Gln, at concentrations much below those present in commercial media, was beneficial to the cell growth. Our approach could prove advantageous in culturing various types of cells.
Seiler, N.; Rebel, G |
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global® allows Day 3 transfer of multiple failure with poor prognosis patients when used instead of co-culture. Clinical trials have shown a 50% pregnancy rate when used for poor prognosis patients and Day 3 replacements in conjuction with Assisted Hatching.1
1 Reference, unplublished data from St. Barnabas Medical Center |
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Fertilization, embryonic development and pregnancy losses with intracytoplasmic sperm injection for surgically-retrieved spermatozoa Anderson, A. R., Wiemer, K. E., Weikert, M. L. & Kyslinger, M. L. Fertilization, embryonic development and pregnancy losses with intracytoplasmic sperm injection for surgically-retrieved spermatozoa. Reprod Biomed Online 5, 142-7 (2002). |
global®
In selected patients the ongoing pregnancy rates following culture in
